Agar Powder

Product Details:

1. Purpose: A highly nutritious general-purpose medium recommended for use when 1% Sodium Chloride is needed in the medium.
2. Composition:
   • Tryptone: 15.0 g/L
   • Soya peptone: 5.0 g/L
   • Sodium chloride: 10.0 g/L
   • Agar: 15.0 g/L
3. Principle:
   • Tryptone and Soya peptone provide nitrogenous compounds and other growth factors.
   • The medium contains a slightly high concentration of sodium chloride, which provides essential ions and maintains osmotic equilibrium.
4. Instructions for Use:
   • Suspend 45.0 grams in 1000 ml purified/distilled water.
   • Heat to boiling to dissolve completely.
   • Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
5. Quality Control Specifications:
   • Appearance of powder: Yellow-colored homogeneous free-flowing powder.
   • Appearance of prepared medium: Yellow-colored clear gel forms in Petri plates.
   • pH (at 25°C): 7.3±0.2
6. Storage: Keep the dehydrated powder in tightly-sealed containers between 25-30°C, protected from direct sunlight. Shelf life is 4 years under optimal conditions.
7. Disposal: Sterilize prepared plates and other contaminated materials before discarding

Product Details:

1. • Purpose:
   • HiCrome™ Listeria Ottaviani Agosti Agar Base is used for the selective and differential isolation of Listeria monocytogenes from food and animal feeds.
   • This medium follows the method adopted by the ISO Committee for Listeria detection 
     • Composition:
   • Contains the chromogenic compound 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside.
   • This substrate detects the presence of β-glucosidase, which is common in all Listeria species.
     •  
       • Appearance: 
     • Listeria colonies appear as blue-green on the medium due to β-glucosidase activity

Product Details:

1. Purpose:

   • EMB agar is a selective and differential culture medium used for the isolation of gram-negative bacilli.
   • It’s commonly used in microbiology labs to identify and differentiate specific bacteria.

2. Composition:

   • Contains:
     • Peptone: Provides essential nutrients for bacterial growth.
     • Lactose: A fermentable carbohydrate.
     • Sucrose: Another fermentable sugar (though not present in all versions).
     • Eosin Y and methylene blue: pH indicator dyes.
     • Agar: Solidifying agent.
     • Dipotassium phosphate: Acts as a buffer.

3. Selective Properties:

   • Inhibits the growth of most gram-positive organisms.
   • Promotes the growth of gram-negative bacteria.

4. Differential Properties:

   • Differentiates bacteria based on their ability to ferment lactose:
     • Lactose fermenters: Produce acid, resulting in dark purple colonies with a green metallic sheen.
     • Slow lactose fermenters: Form brown-pink colonies.
     • Non-lactose fermenters: Produce colorless or light pink colonies.

Product Details:

1.    Purpose: VRBGA is a selective medium used for the detection and enumeration of bile-tolerant Gram-negative bacteria in various samples, including food, water, and other materials of sanitary importance 

2.    Composition:

o    Peptic digest of animal tissue: Provides carbon, nitrogen, vitamins, and other essential growth nutrients.

o    Yeast extract: Additional source of nutrients.

o    Sodium chloride: Maintains osmotic equilibrium.

o    Bile salts mixture: Inhibits accompanying Gram-positive flora.

o    Crystal violet: Further inhibits Gram-positive organisms, especially Staphylococci.

o    Glucose: The fermentable carbohydrate.

o    Neutral red: Acts as a pH indicator 

3.    Result Interpretation:

o    Purple colonies surrounded by purple haloes: Rapid lactose fermenters (e.g., coliform bacteria like E. coli).

o    Pale colonies with greenish zones: Non-fermenters or late lactose fermenters.

o    Pink pin-point colonies: Possibly Enterococci

o    Colorless colonies: Lactose-negative Bacteria 

Product Details:

 

·         Purpose: MSA is a commonly used selective and differential growth medium in microbiology.

·         Selective Action:

·         Contains 7.5% sodium chloride, which selects for bacteria that can tolerate high salt concentrations.

·         Staphylococci species can grow on MSA due to their salt tolerance.

·         Inhibits the growth of most other gram-positive and gram-negative bacteria.

·         Differential Aspect:

·         Staphylococcus aureus (pathogenic staphylococci) can ferment mannitol, changing the pH of the medium to acidic.

·         MSA contains phenol red as a pH indicator.

·         Acidic pH turns the medium yellow due to mannitol fermentation by S. aureus.

·         Coagulase-negative staphylococci (CONS) do not ferment mannitol, so the medium remains pink around their colonies.

·         Composition:

·         Enzymatic digest of casein, animal tissue, and beef extract (sources of nutrients).

·         D-Mannitol as the sole carbohydrate source.

·         Sodium chloride (selective agent).

·         Phenol red (pH indicator).

·         Agar (solidifying agent).

·         Application: Used to isolate and differentiate Staphylococcus spp., particularly S. aureus

 

Product Details:

• • Purpose: Orange Serum Agar is specifically designed for the isolation and enumeration of microorganisms that can survive in citrus products.
  • Microorganisms Targeted: It helps detect acid-tolerant bacteria, molds, and yeasts commonly associated with spoilage in citrus fruit products.
  • Low pH Environment: The low pH of citrus juices limits the growth of microorganisms to those capable of tolerating the acidic conditions.
  • Composition:
    • Tryptone: Provides amino acids and complex nitrogenous substances.
    • Glucose: Serves as a fermentable carbohydrate and energy source.
    • Yeast extract: Supplies vitamins to stimulate growth.
    • Orange serum: Creates an optimal environment for acid-tolerant microorganisms.
    • Superiority: In comparative studies, Orange Serum Agar was found to be superior to other media for total count and detection of spoilage organisms1.
    • Application: Used in laboratories to cultivate and enumerate acidic microorganisms associated with citrus product spoilage

Product Details:

1.    Purpose: Tergitol-7 Agar Base is used for selective enumeration and identification of coliform organisms.

2.    Selective Medium: It helps detect coliform bacteria and allows for early detection of E. coli in water analysis.

3.    Composition: The base contains Sodium heptadecyl sulphate (Tergitol-7), which inhibits gram-positive bacteria.

4.    Modification: Chapman modified the original formula by adding Triphenyl Tetrazolium Chloride (TTC), making the medium selective and differential1.

5.    Recommended Use: Tergitol-7 Agar Base is recommended by the ISO Committee for coliform detection and enumeration

Product Details:

• Purpose: Bismuth Sulphite Agar is a highly selective medium used for the isolation of Salmonella species, particularly S. typhi, from clinical and non-clinical specimens.
• Composition:
  • Peptone: Provides carbon, nitrogen, long-chain amino acids, vitamins, and essential growth factors.
  • HM Peptone B: Another source of nutrients.
  • Dextrose (Glucose): Serves as the carbon source.
  • Disodium phosphate: Maintains osmotic equilibrium.
  • Bismuth sulfite indicator: Inhibits intestinal gram-positive and gram-negative bacteria.
  • Brilliant green: Also inhibits bacterial growth.
  • Agar: Solidifying agent.
• Detection Mechanism:
  • S. Typhi, S. Enteritidis, and S. Typhimurium typically grow as black colonies with a surrounding metallic sheen due to hydrogen sulfide production and reduction of sulfite to black ferric sulfide.
  • Salmonella Paratyphi A grows as light green colonies.
• Preparation:
  • Suspend 52.33 grams in 1000 ml distilled water.
  • Heat to boiling to dissolve the medium completely (do not sterilize in an autoclave).
  • Pour thick plates (25 ml medium per plate) and allow them to solidify.
  • Correctly prepared plates should have a smooth, cream-like opacity with a pale straw color

Product Details:

• Purpose:
  • Simmons Citrate Agar is a differential medium that helps distinguish bacteria based on their ability to utilize citrate as their sole source of carbon.
  • It is commonly used in laboratories to gain insights into the metabolic capabilities of different bacterial species.
• Composition:
  • Contains sodium citrate as the sole carbon source.
  • Ammonium dihydrogen phosphate serves as the sole nitrogen source.
  • Other components include sodium chloride, dipotassium phosphate, magnesium sulfate, bromothy mol blue (pH indicator), and agar (solidifying agent).
• Principle:
  • Organisms capable of utilizing citrate will grow unrestricted on this medium.
  • Bacteria that can metabolize citrate produce an enzyme called citrate-permease, which converts citrate to pyruvate.
  • Pyruvate then enters the organism’s metabolic cycle for energy production.
  • Growth on Simmons Citrate Agar indicates utilization of citrate.
• Uses:
  • Differentiating Gram-negative bacteria based on citrate utilization.
  • Identifying citrate-positive Salmonella species from citrate-negative ones.
  • Aiding in the identification of Entero bacteriaceae.

Product Details:

• Purpose: CCA is a selective agar medium used to detect coliform bacteria in water and other samples.
• Differentiation: It contains chromogenic substrates that allow for distinguishing between coliform and non-coliform bacteria based on their ability to produce specific enzymes.
• Enzyme Indicators:
  • Coliform Bacteria: Produce β-D-galactosidase, resulting in pink to red colonies.
  • E. coli: Produce β-D-glucuronidase, leading to dark blue to violet colonies.
• Composition (per liter):
  • Tryptone: 1 g
  • Yeast extract: 2 g
  • Sodium chloride: 5 g
  • Sodium dihydrogen phosphate: 2.2 g
  • Disodium hydrogen phosphate: 2.7 g
  • Sodium pyruvate: 1 g
  • Sorbitol: 1 g
  • 6-chloro-3-indoxyl β-D-galactopyranoside: 0.2 g
  • 5-bromo-4-chloro-3-indoxyl β-D-glucuronic acid: 0.1 g
  • IPTG (Isopropyl-β-D-thiogalactopyranoside): 0.1 g
  • Agar: 15 g
  • Final pH: 6.8±0.2 at 25°C1.
• Application: Used for microbiological quality testing of water and food samples to detect coliforms

Product Details:

• Purpose: Developed for studying bacteria that inhabit potable water.
• Nutrient Content: Low, which stimulates slow-growing and chlorine-tolerant bacteria.
• Application:
  • Used for qualitative and quantitative heterotrophic plate counts in treated potable water samples.
  • Superior to Plate Count Agar for pour plate, spread plate, or membrane filter techniques.
• Composition:
  • Peptone: Essential amino acids and nitrogen
  • Dextrose: Carbon source
  • Yeast extract: B-complex vitamins and growth enhancer
  • Soluble starch: Neutralizer
  • Sodium pyruvate: Stimulates stressed bacteria growth
  • Magnesium sulfate and dipotassium phosphate: Buffering agents
  • Recommended by: American Public Health Association (APHA) in Standard Methods for Water and Wastewater Examination

Product Details:

• Purpose: Selective isolation of Vibrio cholerae and other enteropathogenic Vibrios
• Composition: Contains peptone, yeast extract, sodium citrate, sodium thiosulfate, sodium cholate, oxgall, sucrose, sodium chloride, ferric citrate, bromothymol blue, thymol blue, and agar
• pH Level: Alkaline pH of 8.8 ± 0.2 to enhance recovery of Vibrio species
• Appearance: Light yellow to light tan homogeneous free-flowing powder
• Preparation: Dissolve in distilled water, heat to boiling, and cool to 50°C before use. Do not autoclave
• Usage: Ideal for laboratory use in microbiology for isolating and identifying Vibrio species

Product Details:

 

·         Chemical Name: Baird Parker Agar Base

·         Use: Selective enumeration medium for Staphylococcus aureus.

·         Composition:

·         Tryptone: Provides nitrogen, carbon, sulfur, and vitamins.

·         HM Peptone B: Source of nutrients.

·         Yeast extract: Supplies carbon, nitrogen, and vitamins.

·         Glycine: Enhances Staphylococcus growth.

·         Sodium pyruvate: Stimulates S. aureus growth.

·         Lithium chloride: Inhibits most contaminants.

·         Agar: Solidifying agent.

·         Principle: Correlates coagulase test with lipolysis zone; black colonies indicate tellurite reduction by coagulase-positive Staphylococci.

·         Result Interpretation: Grey-black colonies and a clear zone are diagnostic for coagulase-positive Staphylococci.

·         Preparation: Dissolve 63.0 grams in 950 ml distilled water, sterilize, and add egg yolk emulsion and potassium tellurite solution

 

Product Details:

Ready-to-use: Convenient sterile powder form for easy preparation.

Nutrient-rich: Supports the growth of a wide variety of microorganisms.

Plant-based: Made from vegetable sources, suitable for various dietary preferences.

Versatile application: Ideal for microbiological culture media in laboratories.

Reliable: Consistent performance for accurate and reproducible results.

Easy storage: Long shelf life when stored properly.

Product Details:

• Purpose: Used for the selective isolation of Pseudomonas species from various samples.
• Composition:
  • Modification of King’s A Medium
  • Contains magnesium chloride and potassium sulfate
• Selective Properties:
  • Promotes pigment production
  • Suppresses growth of Klebsiella, Proteus, and Providencia spp.
• Application Areas:
  • Meat and dairy products
  • Water
  • Environmental samples
  • Clinical specimens

Product Details:

• Purpose: Selective isolation and enumeration of yeasts and molds.
• Composition: Contains yeast extract, glucose, and chloramphenicol.
• Inhibition: Chloramphenicol inhibits bacterial growth.
• Application: Commonly used in dairy product analysis.
• Preparation: Dissolve 40.1 grams in 1 liter of distilled water and sterilize by autoclaving

Product Details:

• Purpose: Enrichment broth for P. aeruginosa.
• Composition:
• DL-Asparagine and L-Proline (nitrogen sources).
• Magnesium sulfate, dipotassium sulfate, and potassium sulfate (mineral components).
• Fluorescent Pigment: Produces a water-soluble, fluorescent pigment in media containing asparagine and ethanol.
• Recommended Technique: Used for cultivating P. aeruginosa via the membrane filter technique.
• BIS Recommendation: Endorsed by the Bureau of Indian Standards

Product Details:

• Purpose: Fraser Broth Base is a liquid medium used with supplements for the selective enrichment of L. monocytogenes and Listeria spp. from food, animal feeding, and environmental samples in the food production and handling area.
• Composition (per liter):
  • Proteose peptone: 5.0 g
  • Tryptone: 5.0 g
  • Lab-Lemco powder: 5.0 g
  • Yeast extract: 5.0 g
  • Sodium chloride: 20.0 g
  • Di-sodium hydrogen phosphate: 12.0 g
  • Potassium dihydrogen phosphate: 1.35 g
  • Aesculin: 1.0 g
  • Lithium chloride: 3.0 g
• pH: 7.2 ± 0.2 at 25°C.
• Supplements:
  • Fraser Supplement: Contains ferric ammonium citrate, nalidixic acid, and acriflavine hydrochloride.
  • Half Fraser Supplement: Used for primary enrichment when combined with Fraser Broth

Product Details:

• Purpose: Used for the selective cultivation of yeasts and molds, especially from clinical and non-clinical samples.
• Composition:
  • Peptones (casein and meat)
  • Dextrose (glucose)
  • Agar
  • Chloramphenicol (antibiotic)
• Preparation:
  • Suspend 65 grams in 1000 ml distilled water.
  • Heat to boiling to dissolve completely.
  • Sterilize by autoclaving at 121°C for 15 minutes.
• Uses:
  • Isolates pathogenic fungi from samples with large numbers of other fungi or bacteria.
  • Commonly used in medical, environmental, and food microbiology.
• Appearance: Clear to slightly opalescent, light amber gel.
• pH: 5.6 ± 0.2 at 25°C

Product Details:

• Purpose: Used for antimicrobial susceptibility testing, particularly with the disk diffusion method
• Composition: Contains beef infusion, casein hydrolysate, starch, and agar
• pH Level: Maintained at 7.3 ± 0.1 at 25°C for optimal microbial growth
• Applications: Suitable for testing a wide range of non-fastidious bacterial pathogens
• Reproducibility: Known for consistent performance and reliable results
• Preparation: Easy to prepare by dissolving in distilled water and sterilizing by autoclaving
• Low Inhibitors: Designed to be low in sulfonamide, trimethoprim, and tetracycline inhibitors
• Standards: Recommended by CLSI (Clinical and Laboratory Standards Institute) for standardized testing

Product Details:

• Definition: Blood Agar Base is a nutrient-rich medium used to grow bacteria, especially those that are fastidious (require specific nutrients).
• Composition: Contains peptone, tryptose, sodium chloride, and agar. Typically enriched with 5% defibrinated mammalian blood (e.g., sheep, horse).
• Purpose:
  • Supports the growth of a wide range of bacteria.
  • Differentiates bacteria based on their hemolytic properties (ability to break down red blood cells).
• Types of Hemolysis:
  • Alpha Hemolysis: Partial hemolysis, greenish discoloration.
  • Beta Hemolysis: Complete hemolysis, clear zone around colonies.
  • Gamma Hemolysis: No hemolysis, no change in the medium.
• Uses:
  • Cultivating fastidious organisms like Streptococcus species.
  • Identifying hemolytic activity of bacteria.
• Preparation:
  • Dissolve the base in water, autoclave, cool, and add sterile blood.
  • Pour into sterile plates for use.

Product Details:

Chromogenic Coliform Agar (CCA) by HiMedia is a selective and differential medium recommended by ISO 9308-1 for the simultaneous detection and enumeration of Escherichia coli and coliform bacteria in water samples. It utilizes chromogenic substrates that enable easy differentiation between E. coli and other coliforms based on colony color, eliminating the need for confirmatory tests.

Product Details:

Chloramphenicol Yeast Glucose Agar is a selective medium used for the isolation and enumeration of yeasts and molds in various samples, especially in food, water, and clinical specimens. The medium contains chloramphenicol, an antibiotic that suppresses bacterial growth, allowing fungi to grow without interference.

Product Details:

MacConkey Agar is a selective and differential medium developed for the isolation and differentiation of Gram-negative enteric bacilli, especially the Enterobacteriaceae family based on lactose fermentation. It is widely used in clinical, food, water, and pharmaceutical microbiology.

 

Product Details:

Sterile Fluid Thioglycollate Medium is a ready-to-use sterility test medium, prepared in accordance with pharmacopoeial standards including USP, EP, BP, and JP. It is primarily used for the sterility testing of pharmaceutical products and medical devices.

Product Details:

Immersion oil is a specially formulated optical medium used in microscopy to increase the resolving power of a microscope. By having a refractive index similar to glass, it minimizes light refraction between the slide and objective lens, thereby providing sharper, clearer images at high magnifications.

Product Details:

• Purpose:
  • BHI Agar is a general-purpose medium used for the primary isolation of aerobic bacteria from clinical specimens.
  • It is highly nutritious and can support luxuriant growth of a wide variety of microorganisms.
• Composition:
  • BHI Agar derives its nutrients from brain heart infusion, peptone, and glucose components.
  • Protease peptone and infusions serve as sources of carbon, nitrogen, vitamins, amino acids, and essential growth factors.
• Enrichment and Selectivity:
  • BHI Agar can be further enriched by the addition of blood.
  • It can also be rendered selective by adding different antibiotics.
• Applications:
  • Cultivation of fastidious pathogenic bacteria, yeasts, and moulds.
  • Widely used in clinical laboratories for bacterial isolation

Product Details:

             Purpose: Recommended for enrichment of Salmonellae, based on its ability to multiply selectively at high osmotic pressure, low pH, and at 43°C, with modest nutritional requirements

·         Composition: Contains aluminum oxide (Al₂O₃) with impurities.

·         Applications:

o    Chromatography: Used as a buffer substance for chromatography in analytical applications.

o    Polishing: Suitable for surface finishing in ceramics, glass, and metals industries.

o    Abrasive: Effective for grinding, cutting, and shaping hard materials.

o    Catalyst Support: Used as a support material in catalysts.

·         Chemical Formula: Na₂HPO₄ · 2 H₂O

·         Molecular Weight: 177.99 g/mol

·         Density: Varies based on particle size.

·         Storage: Keep in a dry, cool place away from moisture.

Product Details:

1.    Purpose: Lactobacillus MRS Agar is a medium used for the selective isolation and enumeration of Lactobacilli from various sources, including feces and dairy.

2.    Composition:

o    Proteose Peptone, Beef Extract, and Yeast Extract provide nitrogenous sources, carbohydrates, vitamins, and minerals.

o    Glucose serves as the primary carbohydrate.

o    Other components include dipotassium phosphate, sodium acetate, triammonium citrate, magnesium sulfate, manganese sulfate, Tween® 80, and agar.

3.    Development: Originally developed in 1960 by de Man, Rogosa & Sharpe, this medium is suitable for most lactic acid bacteria and serves as a substitute for Tomato Juice Agar.

4.    pH Adjustment: When acidified to pH 5.4, it can be used to enumerate Lactobacillus bulgaricus in yogurts.

5.    Selectivity: Ammonium citrate and sodium acetate provide selectivity against streptococci and molds.

6.    Incubation: Incubate agar plates at 35°C for 3 days or at 30°C for 5 days in an aerobic atmosphere supplemented with carbon dioxide

Product Details:

• Purpose: MacConkey broth is a selective culture medium containing lactose.
• Function:
• Lactose degradation by bacteria produces acid and gas.
• Gas is collected in Durham tubes.
• A color change of the pH indicator bromocresol purple to yellow indicates acid production.
• Uses:
• Detection of coliform bacteria in milk and water.
• Selective enrichment of Escherichia coli from non-sterile samples

 

Product Details:

• Purpose: Selective and differential medium for isolating Gram-negative bacteria
• Applications: Commonly used to identify and differentiate Enterobacteriaceae family members
• Indicator: Neutral red (turns pink/red with lactose fermentation)
• Components: Peptone, lactose, bile salts, sodium chloride, neutral red, crystal violet, agar
• Preparation: Dissolve 49.53g in 1L distilled water, sterilize by autoclaving
• Storage: Store prepared plates at 2-8°C
• pH: Final pH of 7.1 ± 0.2 at 25°C
• Colony Appearance: Lactose fermenters appear pink/red; non-fermenters remain colorless

Product Details:

• Purpose: Differential medium for detecting coliforms
• Applications: Used in water and milk testing
• Indicator: Bromocresol purple (changes from purple to yellow with acid production)
• Components: Peptone, lactose, bile salts, sodium chloride
• Preparation: Dissolve 40g in 1L distilled water, sterilize by autoclaving
• Storage: Store prepared media below 8°C, dehydrated powder at 2-25°C
• pH: Final pH of 7.4 ± 0.2 at 25°C

Product Details:

• Composition:
  • Agar: 20.0 g/L
  • Cupric sulfate: 0.4 g/L
  • Dextrose: 10.0 g/L
  • Gelatin peptone: 5.0 g/L
  • Malt extract: 3.0 g/L
  • Yeast extract: 3.0 g/L
• pH Level: Final pH of 6.2 ± 0.2 at 25°C
• Form: Powder
• Application:
  • Selective medium for isolating and cultivating wild yeasts in the brewing industry
• Preparation:
  • Suspend 41.4 grams in 1000 ml distilled water.
  • Heat to boiling to dissolve completely.
  • Sterilize by autoclaving at 121°C for 15 minutes.
  • Pour into sterile Petri plates.

Product Details:

• Definition: ECD Agar is a selective medium used for detecting coliforms, especially Escherichia coli, in water, food, and other samples.
• Composition: Contains tryptone, yeast extract, bile salts, sodium chloride, phosphate salts, and agar.
• Purpose:
  • Selectively isolates coliform bacteria.
  • Differentiates E. coli based on indole production.
• Usage:
  • Commonly used in water testing and food safety analysis.
  • Utilized in membrane filter techniques.
• Preparation:
  • Dissolve the medium in water, autoclave, cool, and pour into sterile Petri plates.
  • Incubate samples on the medium and observe for colony growth.
• Advantages:
  • Selective: Inhibits non-coliform bacteria, ensuring accurate results.
  • Differential: Identifies E. coli through indole reaction (red zone around colonies).
  • Reliable: Provides consistent and reproducible results.
• Applications:
  • Monitoring water quality.
  • Ensuring food safety.
  • Conducting microbiological research.

Product Details:

Lactobacillus MRS Agar is a nutrient-rich, selective medium formulated by de Man, Rogosa and Sharpe (MRS) for the cultivation, enumeration, and isolation of Lactobacilli and other lactic acid bacteria from various samples including dairy, food, and probiotic products.

 

Product Details:

Sterile Soyabean Casein Digest Medium (also known as Tryptic Soy Broth or TSB) is a general-purpose medium used for the sterility testing of pharmaceutical products, medical devices, and biological products, in accordance with USP, EP, BP, and JP standards.

 

Product Details:

1. Chemical Name: Shigella Broth Base
2. Use: Isolation and cultivation of Shigella species from food
3. Composition:
   • Contains casein enzymic hydrolysate as a source of carbon, nitrogen, vitamins, and minerals
   • Dextrose provides necessary carbohydrates
   • Buffering by dipotassium hydrogen phosphate and potassium dihydrogen phosphate
   • Sodium chloride maintains osmotic balance of the medium
4. Safety: Handle with care; avoid contact with skin and eyes

Product Details:

·         Chemical Name: BHI Broth (Brain Heart Infusion Broth)

·         Use: Highly nutritive medium for cultivating a wide variety of microorganisms.

·         Applications:

o   Preparation of inocula for antimicrobial susceptibility testing.

o   Preferred medium for anaerobic bacteria, yeasts, and other microorganisms.

·         Composition:

o   Contains casein enzymic hydrolysate, dextrose, and buffering agents.

·         Safety: Handle with care; avoid contact with skin and eyes.

·         Storage: Store appropriately to maintain stability

Product Details:

Plate Count Agar, also known as Standard Methods Agar, is a general-purpose medium used for enumerating viable microorganisms in milk, food, water, and other materials. It supports the growth of a wide variety of bacteria and is widely used in routine microbial count testing.

Product Details:

• Type: Cationic dye (positively charged)
• Color: Blue
• Usage: Staining bacteria, fungi, and other microorganisms
• Applications: Microbiology, histology, and cytology
• Function: Binds to negatively charged cell components like DNA and RNA
• Preparation: Dissolve in distilled water for use
• Storage: Keep in a dark, cool place to maintain efficacy
• Certifications: Often certified by the Biological Stain Commission