Agar Powder

Product Details:

1. Purpose: A highly nutritious general-purpose medium recommended for use when 1% Sodium Chloride is needed in the medium.
2. Composition:
   • Tryptone: 15.0 g/L
   • Soya peptone: 5.0 g/L
   • Sodium chloride: 10.0 g/L
   • Agar: 15.0 g/L
3. Principle:
   • Tryptone and Soya peptone provide nitrogenous compounds and other growth factors.
   • The medium contains a slightly high concentration of sodium chloride, which provides essential ions and maintains osmotic equilibrium.
4. Instructions for Use:
   • Suspend 45.0 grams in 1000 ml purified/distilled water.
   • Heat to boiling to dissolve completely.
   • Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
5. Quality Control Specifications:
   • Appearance of powder: Yellow-colored homogeneous free-flowing powder.
   • Appearance of prepared medium: Yellow-colored clear gel forms in Petri plates.
   • pH (at 25°C): 7.3±0.2
6. Storage: Keep the dehydrated powder in tightly-sealed containers between 25-30°C, protected from direct sunlight. Shelf life is 4 years under optimal conditions.
7. Disposal: Sterilize prepared plates and other contaminated materials before discarding

Product Details:

             Purpose: Recommended for enrichment of Salmonellae, based on its ability to multiply selectively at high osmotic pressure, low pH, and at 43°C, with modest nutritional requirements

·         Composition: Contains aluminum oxide (Al₂O₃) with impurities.

·         Applications:

o    Chromatography: Used as a buffer substance for chromatography in analytical applications.

o    Polishing: Suitable for surface finishing in ceramics, glass, and metals industries.

o    Abrasive: Effective for grinding, cutting, and shaping hard materials.

o    Catalyst Support: Used as a support material in catalysts.

·         Chemical Formula: Na₂HPO₄ · 2 H₂O

·         Molecular Weight: 177.99 g/mol

·         Density: Varies based on particle size.

·         Storage: Keep in a dry, cool place away from moisture.

Product Details:

1. • Purpose:
   • HiCrome™ Listeria Ottaviani Agosti Agar Base is used for the selective and differential isolation of Listeria monocytogenes from food and animal feeds.
   • This medium follows the method adopted by the ISO Committee for Listeria detection 
     • Composition:
   • Contains the chromogenic compound 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside.
   • This substrate detects the presence of β-glucosidase, which is common in all Listeria species.
     •  
       • Appearance: 
     • Listeria colonies appear as blue-green on the medium due to β-glucosidase activity

Product Details:

1. Purpose:

   • EMB agar is a selective and differential culture medium used for the isolation of gram-negative bacilli.
   • It’s commonly used in microbiology labs to identify and differentiate specific bacteria.

2. Composition:

   • Contains:
     • Peptone: Provides essential nutrients for bacterial growth.
     • Lactose: A fermentable carbohydrate.
     • Sucrose: Another fermentable sugar (though not present in all versions).
     • Eosin Y and methylene blue: pH indicator dyes.
     • Agar: Solidifying agent.
     • Dipotassium phosphate: Acts as a buffer.

3. Selective Properties:

   • Inhibits the growth of most gram-positive organisms.
   • Promotes the growth of gram-negative bacteria.

4. Differential Properties:

   • Differentiates bacteria based on their ability to ferment lactose:
     • Lactose fermenters: Produce acid, resulting in dark purple colonies with a green metallic sheen.
     • Slow lactose fermenters: Form brown-pink colonies.
     • Non-lactose fermenters: Produce colorless or light pink colonies.

Product Details:

1.    Purpose: VRBGA is a selective medium used for the detection and enumeration of bile-tolerant Gram-negative bacteria in various samples, including food, water, and other materials of sanitary importance 

2.    Composition:

o    Peptic digest of animal tissue: Provides carbon, nitrogen, vitamins, and other essential growth nutrients.

o    Yeast extract: Additional source of nutrients.

o    Sodium chloride: Maintains osmotic equilibrium.

o    Bile salts mixture: Inhibits accompanying Gram-positive flora.

o    Crystal violet: Further inhibits Gram-positive organisms, especially Staphylococci.

o    Glucose: The fermentable carbohydrate.

o    Neutral red: Acts as a pH indicator 

3.    Result Interpretation:

o    Purple colonies surrounded by purple haloes: Rapid lactose fermenters (e.g., coliform bacteria like E. coli).

o    Pale colonies with greenish zones: Non-fermenters or late lactose fermenters.

o    Pink pin-point colonies: Possibly Enterococci

o    Colorless colonies: Lactose-negative Bacteria 

Product Details:

·         Purpose: MSA is a commonly used selective and differential growth medium in microbiology.

·         Selective Action:

·         Contains 7.5% sodium chloride, which selects for bacteria that can tolerate high salt concentrations.

·         Staphylococci species can grow on MSA due to their salt tolerance.

·         Inhibits the growth of most other gram-positive and gram-negative bacteria.

·         Differential Aspect:

·         Staphylococcus aureus (pathogenic staphylococci) can ferment mannitol, changing the pH of the medium to acidic.

·         MSA contains phenol red as a pH indicator.

·         Acidic pH turns the medium yellow due to mannitol fermentation by S. aureus.

·         Coagulase-negative staphylococci (CONS) do not ferment mannitol, so the medium remains pink around their colonies.

·         Composition:

·         Enzymatic digest of casein, animal tissue, and beef extract (sources of nutrients).

·         D-Mannitol as the sole carbohydrate source.

·         Sodium chloride (selective agent).

·         Phenol red (pH indicator).

·         Agar (solidifying agent).

·         Application: Used to isolate and differentiate Staphylococcus spp., particularly S. aureus

Product Details:

• • Purpose: Orange Serum Agar is specifically designed for the isolation and enumeration of microorganisms that can survive in citrus products.
  • Microorganisms Targeted: It helps detect acid-tolerant bacteria, molds, and yeasts commonly associated with spoilage in citrus fruit products.
  • Low pH Environment: The low pH of citrus juices limits the growth of microorganisms to those capable of tolerating the acidic conditions.
  • Composition:
    • Tryptone: Provides amino acids and complex nitrogenous substances.
    • Glucose: Serves as a fermentable carbohydrate and energy source.
    • Yeast extract: Supplies vitamins to stimulate growth.
    • Orange serum: Creates an optimal environment for acid-tolerant microorganisms.
    • Superiority: In comparative studies, Orange Serum Agar was found to be superior to other media for total count and detection of spoilage organisms1.
    • Application: Used in laboratories to cultivate and enumerate acidic microorganisms associated with citrus product spoilage

Product Details:

1.    Purpose: Tergitol-7 Agar Base is used for selective enumeration and identification of coliform organisms.

2.    Selective Medium: It helps detect coliform bacteria and allows for early detection of E. coli in water analysis.

3.    Composition: The base contains Sodium heptadecyl sulphate (Tergitol-7), which inhibits gram-positive bacteria.

4.    Modification: Chapman modified the original formula by adding Triphenyl Tetrazolium Chloride (TTC), making the medium selective and differential1.

5.    Recommended Use: Tergitol-7 Agar Base is recommended by the ISO Committee for coliform detection and enumeration

Product Details:

• Purpose: Bismuth Sulphite Agar is a highly selective medium used for the isolation of Salmonella species, particularly S. typhi, from clinical and non-clinical specimens.
• Composition:
  • Peptone: Provides carbon, nitrogen, long-chain amino acids, vitamins, and essential growth factors.
  • HM Peptone B: Another source of nutrients.
  • Dextrose (Glucose): Serves as the carbon source.
  • Disodium phosphate: Maintains osmotic equilibrium.
  • Bismuth sulfite indicator: Inhibits intestinal gram-positive and gram-negative bacteria.
  • Brilliant green: Also inhibits bacterial growth.
  • Agar: Solidifying agent.
• Detection Mechanism:
  • S. Typhi, S. Enteritidis, and S. Typhimurium typically grow as black colonies with a surrounding metallic sheen due to hydrogen sulfide production and reduction of sulfite to black ferric sulfide.
  • Salmonella Paratyphi A grows as light green colonies.
• Preparation:
  • Suspend 52.33 grams in 1000 ml distilled water.
  • Heat to boiling to dissolve the medium completely (do not sterilize in an autoclave).
  • Pour thick plates (25 ml medium per plate) and allow them to solidify.
  • Correctly prepared plates should have a smooth, cream-like opacity with a pale straw color

Product Details:

• Purpose:
  • Simmons Citrate Agar is a differential medium that helps distinguish bacteria based on their ability to utilize citrate as their sole source of carbon.
  • It is commonly used in laboratories to gain insights into the metabolic capabilities of different bacterial species.
• Composition:
  • Contains sodium citrate as the sole carbon source.
  • Ammonium dihydrogen phosphate serves as the sole nitrogen source.
  • Other components include sodium chloride, dipotassium phosphate, magnesium sulfate, bromothy mol blue (pH indicator), and agar (solidifying agent).
• Principle:
  • Organisms capable of utilizing citrate will grow unrestricted on this medium.
  • Bacteria that can metabolize citrate produce an enzyme called citrate-permease, which converts citrate to pyruvate.
  • Pyruvate then enters the organism’s metabolic cycle for energy production.
  • Growth on Simmons Citrate Agar indicates utilization of citrate.
• Uses:
  • Differentiating Gram-negative bacteria based on citrate utilization.
  • Identifying citrate-positive Salmonella species from citrate-negative ones.
  • Aiding in the identification of Entero bacteriaceae.

Product Details:

• Purpose: CCA is a selective agar medium used to detect coliform bacteria in water and other samples.
• Differentiation: It contains chromogenic substrates that allow for distinguishing between coliform and non-coliform bacteria based on their ability to produce specific enzymes.
• Enzyme Indicators:
  • Coliform Bacteria: Produce β-D-galactosidase, resulting in pink to red colonies.
  • E. coli: Produce β-D-glucuronidase, leading to dark blue to violet colonies.
• Composition (per liter):
  • Tryptone: 1 g
  • Yeast extract: 2 g
  • Sodium chloride: 5 g
  • Sodium dihydrogen phosphate: 2.2 g
  • Disodium hydrogen phosphate: 2.7 g
  • Sodium pyruvate: 1 g
  • Sorbitol: 1 g
  • 6-chloro-3-indoxyl β-D-galactopyranoside: 0.2 g
  • 5-bromo-4-chloro-3-indoxyl β-D-glucuronic acid: 0.1 g
  • IPTG (Isopropyl-β-D-thiogalactopyranoside): 0.1 g
  • Agar: 15 g
  • Final pH: 6.8±0.2 at 25°C1.
• Application: Used for microbiological quality testing of water and food samples to detect coliforms

Product Details:

• Purpose:
  • BHI Agar is a general-purpose medium used for the primary isolation of aerobic bacteria from clinical specimens.
  • It is highly nutritious and can support luxuriant growth of a wide variety of microorganisms.
• Composition:
  • BHI Agar derives its nutrients from brain heart infusion, peptone, and glucose components.
  • Protease peptone and infusions serve as sources of carbon, nitrogen, vitamins, amino acids, and essential growth factors.
• Enrichment and Selectivity:
  • BHI Agar can be further enriched by the addition of blood.
  • It can also be rendered selective by adding different antibiotics.
• Applications:
  • Cultivation of fastidious pathogenic bacteria, yeasts, and moulds.
  • Widely used in clinical laboratories for bacterial isolation

Product Details:

1. Chemical Name: Shigella Broth Base
2. Use: Isolation and cultivation of Shigella species from food
3. Composition:
   • Contains casein enzymic hydrolysate as a source of carbon, nitrogen, vitamins, and minerals
   • Dextrose provides necessary carbohydrates
   • Buffering by dipotassium hydrogen phosphate and potassium dihydrogen phosphate
   • Sodium chloride maintains osmotic balance of the medium
4. Safety: Handle with care; avoid contact with skin and eyes

Product Details:

·         Chemical Name: Baird Parker Agar Base

·         Use: Selective enumeration medium for Staphylococcus aureus.

·         Composition:

·         Tryptone: Provides nitrogen, carbon, sulfur, and vitamins.

·         HM Peptone B: Source of nutrients.

·         Yeast extract: Supplies carbon, nitrogen, and vitamins.

·         Glycine: Enhances Staphylococcus growth.

·         Sodium pyruvate: Stimulates S. aureus growth.

·         Lithium chloride: Inhibits most contaminants.

·         Agar: Solidifying agent.

·         Principle: Correlates coagulase test with lipolysis zone; black colonies indicate tellurite reduction by coagulase-positive Staphylococci.

·         Result Interpretation: Grey-black colonies and a clear zone are diagnostic for coagulase-positive Staphylococci.

·         Preparation: Dissolve 63.0 grams in 950 ml distilled water, sterilize, and add egg yolk emulsion and potassium tellurite solution

Product Details:

·         Chemical Name: BHI Broth (Brain Heart Infusion Broth)

·         Use: Highly nutritive medium for cultivating a wide variety of microorganisms.

·         Applications:

o   Preparation of inocula for antimicrobial susceptibility testing.

o   Preferred medium for anaerobic bacteria, yeasts, and other microorganisms.

·         Composition:

o   Contains casein enzymic hydrolysate, dextrose, and buffering agents.

·         Safety: Handle with care; avoid contact with skin and eyes.

·         Storage: Store appropriately to maintain stability

Product Details:

• Purpose: Buffered Sodium Chloride Peptone Solution is primarily used for microbial limit testing in pharmaceutical products.
• Composition: The solution contains sodium chloride, peptone, and a phosphate buffer. The low peptone content helps maintain the viability of microorganisms without supporting their growth 
• Applications:
  • Microbial Examination: Used for the microbial examination of non-sterile pharmaceutical products 
  • Sample Preparation: Can be used as a solution for sample preparation, dilution, or washing
  • Cosmetics: Conforms to ISO 21149 for the detection of viable microorganisms in cosmetics

Product Details:

1.    Purpose: Lactobacillus MRS Agar is a medium used for the selective isolation and enumeration of Lactobacilli from various sources, including feces and dairy.

2.    Composition:

o    Proteose Peptone, Beef Extract, and Yeast Extract provide nitrogenous sources, carbohydrates, vitamins, and minerals.

o    Glucose serves as the primary carbohydrate.

o    Other components include dipotassium phosphate, sodium acetate, triammonium citrate, magnesium sulfate, manganese sulfate, Tween® 80, and agar.

3.    Development: Originally developed in 1960 by de Man, Rogosa & Sharpe, this medium is suitable for most lactic acid bacteria and serves as a substitute for Tomato Juice Agar.

4.    pH Adjustment: When acidified to pH 5.4, it can be used to enumerate Lactobacillus bulgaricus in yogurts.

5.    Selectivity: Ammonium citrate and sodium acetate provide selectivity against streptococci and molds.

6.    Incubation: Incubate agar plates at 35°C for 3 days or at 30°C for 5 days in an aerobic atmosphere supplemented with carbon dioxide

Product Details:

• Purpose:
  • PDA is a frequently used microbial growth medium for the cultivation of molds, yeasts, and other fungi.
  • It provides a rich nutritional base that encourages fungal growth.
• Composition:
  • Potato Infusion: Derived from boiled potatoes, it serves as a nutrient source.
  • Dextrose (Glucose): A fermentable carbohydrate.
  • Agar: Added as the solidifying agent.
• Selective Properties:
  • PDA can be supplemented with acid or antibiotics to inhibit bacterial growth.
  • It selectively promotes the growth of fungi.
• Uses:
  • Detection of yeasts and molds in dairy products and prepared foods.
  • Cultivation of yeasts and molds from clinical specimens.
  • Recommended for plate count methods in food testing.
• Preparation:
  • Prepare potato infusion by boiling sliced, unpeeled potatoes in water.
  • Mix with dextrose, agar, and water, then autoclave.
  • Dispense into sterile petri dishes.
• Variants:
  • PDA with TA (Tartaric Acid) inhibits bacterial growth.
  • PDA with Chlortetracycline is used for microbial enumeration in cosmetics.
  • PDA with Chloramphenicol selectively cultivates fungi from mixed samples

Product Details:

1.       Purpose: RC Agar is an enriched non-selective medium used for the cultivation of Clostridia and other anaerobic and facultative bacteria from various clinical and non-clinical specimens

   2.      Composition: It is a solid, highly nutritious medium containing ingredients that support the growth of anaerobes, lactobacilli, and other bacterial species.

   3.      Applications:

   o    Isolation and cultivation of anaerobic organisms from different habitats, including food and pathological samples.

   o    Enumeration of clostridia and other anaerobes.

   4.      Performance: RC Agar performs similarly to the pork-starch-pea agar for anaerobe counts.

   5.      Additional Uses:

   o    Investigation of intestinal flora in humans and animals.

   o    Anaerobic count of microorganisms from soil samples.

   6.      Preparation: RC Agar is available in dehydrated form and can be reconstituted with water before use

Product Details:

• Purpose: Bismuth Sulphite Agar is a highly selective medium used for the isolation of Salmonella species, particularly S. typhi, from clinical and non-clinical specimens.
• Composition:
  • Peptone: Provides carbon, nitrogen, long-chain amino acids, vitamins, and essential growth factors.
  • HM Peptone B: Another source of nutrients.
  • Dextrose (Glucose): Serves as the carbon source.
  • Disodium phosphate: Maintains osmotic equilibrium.
  • Bismuth sulfite indicator: Inhibits intestinal gram-positive and gram-negative bacteria.
  • Brilliant green: Also inhibits bacterial growth.
  • Agar: Solidifying agent.
• Preparation:
  • Suspend 52.33 grams in 1000 ml distilled water.
  • Heat to boiling to dissolve the medium completely (do not sterilize in an autoclave).
  • Pour thick plates (25 ml medium per plate) and allow them to solidify.
  • Correctly prepared plates should have a smooth, cream-like opacity with a pale straw color